{"id":384,"date":"2015-06-10T08:08:42","date_gmt":"2015-06-10T07:08:42","guid":{"rendered":"http:\/\/wp.cs.ucl.ac.uk\/gift-surg-intranet\/?p=384"},"modified":"2015-06-10T08:08:42","modified_gmt":"2015-06-10T07:08:42","slug":"fetal-sheep-model-of-spina-bifida-may-2015","status":"publish","type":"post","link":"https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/2015\/06\/10\/fetal-sheep-model-of-spina-bifida-may-2015\/","title":{"rendered":"fetal sheep model of spina bifida-May 2015"},"content":{"rendered":"<p>With my colleague Alexander Engels, we are improving the readouts of the fetal lamb model of open spina bifida:<\/p>\n<ul>\n<li>Analysis of watertightness of lesion: just prior to delivery an amniocentesis was performed to test for AcetylCholinesterase and alpha-FetoProtein in the amniotic fluid (should be negative with complete closure): unfortunately the results were not conclusive as these human markers could not be measured in the amniotic fluid.<\/li>\n<li>Brain and spine MRI under general anesthesia using the 3-Tesla SIEMENS MAGNETOM TrioTim syngo MR B17 machine. We analyzed anatomical (T1, T2 and T2 flair) and watertightness (T2-Turbo Inversed Recovery (TIR) sequences) of SB (fig.2-4).<\/li>\n<li>Histological examination of the operated lumbar spine and of the brain (Meuli-1995): after euthanasia of the lambs, the whole blood was removed via incision of the inferior vena cava. Then we performed a whole-body vascular rinsing with 3L of saline through the abdominal aorta and whole-body vascular fixation with infusion of 3L of the 4% paraformaldehyde. Subsequently the entire spine and the brain were harvested <em>en bloc<\/em> and fixed for a month in a container full of 4% paraformaldehyde. Serial sagittal and cross sections of the spina and the brain are being prepared and stained with Hematoxylin-Eosin and Gomori\u2019s trichrome (<a href=\"#_ENREF_1\">Meuli, Meuli-Simmen et al. 1995<\/a>).<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p><em>Figure 2 &#8211; T2 sagittal view of the spinal cord at the level of the lumbar defect.<\/em><\/p>\n<p><a href=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.27.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone size-medium wp-image-526\" src=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.27-300x248.png\" alt=\"Screen Shot 2015-06-03 at 15.48.27\" width=\"300\" height=\"248\" srcset=\"https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.27-300x248.png 300w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.27-250x206.png 250w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.27.png 532w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><\/p>\n<p><em>Figure 3 &#8211; TSI transversal view of the spinal cord at the level of the defect showing a leakage of cerebrospinal fluid.<\/em><\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.32.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-525\" src=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.32-300x151.png\" alt=\"Screen Shot 2015-06-03 at 15.48.32\" width=\"497\" height=\"250\" srcset=\"https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.32-300x151.png 300w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.32-250x126.png 250w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.32.png 678w\" sizes=\"auto, (max-width: 497px) 100vw, 497px\" \/><\/a><\/p>\n<p><em>Figure 4 \u2013 T2 sagittal view of the brain and brainstem of a spina bifida neonatal sheep showing a Chiari 2 malformation (hindbrain herniation) (above) compared to the same view of a normal neonatal sheep (below).<\/em><\/p>\n<p><em>\u00a0<\/em><\/p>\n<p><a href=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.43.png\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone  wp-image-524\" src=\"http:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.43-267x300.png\" alt=\"Screen Shot 2015-06-03 at 15.48.43\" width=\"348\" height=\"391\" srcset=\"https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.43-267x300.png 267w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.43-250x281.png 250w, https:\/\/wp1.cs.ucl.ac.uk\/gift-surg-intranet\/wp-content\/uploads\/sites\/14\/2015\/06\/Screen-Shot-2015-06-03-at-15.48.43.png 629w\" sizes=\"auto, (max-width: 348px) 100vw, 348px\" \/><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>With my colleague Alexander Engels, we are improving the readouts of the fetal lamb model of open spina bifida: Analysis of watertightness of lesion: just prior to delivery an amniocentesis was performed to test for AcetylCholinesterase and alpha-FetoProtein in the<\/p>\n","protected":false},"author":35,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[4],"tags":[],"class_list":["post-384","post","type-post","status-publish","format-standard","hentry","category-electronic-journal"],"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.3 - 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